mature.rest
Mature vs. Rest. It is an interesting debate that goes back and forth in many online communities. Indeed, HATETOP/REST vs. Mature. When people argue over the question of whether or not Mature API's are necessary or even helpful, they usually never stop to think about what Mature actually is.

When speaking of "restorative", people usually mean Nurture and Clarity. That is, to rest and nurture a living cell. To do this, the cell must be kept in a state of perfect stasis. That is, it must remain uncluttered and free of any cellular debris. Furthermore, there must be no inputs (stimulated by hormones or other means) to the cell, or it will die.

Hormones are the key to keeping cells in a state of rest. But, we can artificially induce this state in either human or animal stem cells. To do this, we need to turn them into specific cell types. In essence, we turn these specific cell types into "stored" states. Stored cell types can then be "re-energized" and brought back to their "potential" state by adding chromatin.

The question of whether or not a Mature-based stem cell rejuvenates is not easily answered, but I think that answering it should be made easier. Mature stem cells have a set of genes (called MIRs) that respond only to a certain set of stimuli. Therefore, if we add specific MIR genes, and those genes are activated, a cell will be generated that will live and grow into an adult. That's my take on it. However, I believe that further research should be done first.

There are several candidates for activating MIRs, including: transcription factors, developmental regulators, growth hormone, insulin, and neurotensin. It appears that the pancreatic islet cells receive MIR-related signals and activate. Other potential candidates are: VHf1/phosphatidyl choline, sirtuins and enhancers. In conclusion, our results strongly suggest that the new studies should focus on the genes that are MIR-related. This will allow researchers to isolate and discover which genes are important for activating MIRs, thus bringing us one step closer to developing a Mature-biased somatotrope.

One issue that still remains is whether or not the addition of a MIR-related developmental regulator will change the stability of the gene, or will it change its activity. I believe that it will increase the stability and decrease the activity. The increased stability will allow the gene to be expressed longer in the developing nervous system. The decreased activity, however, will likely only be seen with cells that are already mature. So, will we see an effect of adding a developmental regulator in the developing nervous system, or will the effects of adding MIRs only occur with cells that are already mature?

I think it's more likely that future findings will show that genes activated by MIRs are stable. This will likely mean that the effects of MIRs will be more significant in human embryos than in flies or worms. But, if true, this could have implications for future treatments. For example, some cancers can be treated by blocking the tumor cells from using the beta cells in the nucleus. In the case of MIR-related genes, if the gene regulatory proteins that act as MIRs are unstable, the cancer cells may still use the beta cell for their growth, even though the cancer has spread to other parts of the body.

There are some interesting implications here, but it's important to remember that all of these things are very speculative. As exciting as some of these technologies are, the reality is that we don't know enough about the MIRs and the proteins they stimulate to make broad statements. Until someone releases a comprehensive study that describes the effects of adding MIRs to either the developing or adult stem cells, there will always be a lot of guesswork involved. But, it looks as if we may finally be on the cusp of treating all different types of diseases and disorders with the application of stem cells, whether they are from embryos or adults. This is certainly a fascinating area of research, and it looks as if the future of disease prevention may indeed be in chromatin regulation.

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